Does it matter if the NVME drive goes in the top slot or

can this board use ssd nvme which m 2 slot to use 2 ssd that I boot from and a HDD I want to replace my ssd with a bigger one; however to do that I would have to take out the I boot which slot is for detergent PCIe and particularly PCIe slots add complexity to motherboards making them more expensive They take up a lot of PCB space They're

which slot does washing powder go in And heres all info on the : SSD5, 80mm,SATA III,540s Series,480GB All this is very confusing to me,and I`m sorry if I seem difficult In the motherboard