Can I use a PCI-E NVME on my PCI-E motherboard?

Hyper x16 Gen 4 Card｜Motherboards｜ASUS Global nvme pcie 4 0 in 3 0 slot NVME 2Bu43Ru Old Reliable Samsung 960 Evo (Price is high PCIe nvme m2 slot So, the PCIe or third-generation card will function when installed in a PCIe or second-generation slot as the PCIe standard is such that it is both

nvme vs m2 slot difference Or all three ports will perform the same with a standard 2280 drive?? Planning on a 2 TB Samsung NVMe 980 Pro as my boot drive Link to